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KMID : 1377020210180061009
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Tissue Engineering and Regenerative Medicine
2021 Volume.18 No. 6 p.1009 ~ p.1020
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Polydeoxyribonucleotide and Polynucleotide Improve Tendon Healing and Decrease Fatty Degeneration in a Rat Cuff Repair Model
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Hwang Jung-Taek
Lee Sang-Soo
Han Sang-Hak
Sherchan Binod
Panakkal Jiss Joseph
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Abstract
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Background: After surgical repair of chronic rotator cuff tears, healing of the repaired tendons often fails and is accompanied by high-level fatty degeneration. Our purpose was to explore the effects of polydeoxyribonucleotide (PDRN) and polynucleotide (PN) on tendon healing and the reversal of fatty degeneration in a chronic rotator cuff tear model using a rat infraspinatus.
Methods: Sixty rats were randomly assigned to the following three groups (20 rats per group: 12 for histological evaluation and 8 for mechanical testing): saline?+?repair (SR), PDRN?+?repair (PR), and PN?+?repair (PNR). The right shoulder was used for experimental intervention, and the left served as a control. Four weeks after detaching the infraspinatus, the torn tendon was repaired. Saline, PDRN, and PN were applied to the repair sites. Histological evaluation was performed 3 and 6 weeks after repair and biomechanical analysis was performed at 6 weeks.
Results: Three weeks after repair, the PR and PNR groups had more CD168-stained cells than the SR group. The PR group showed a larger cross-sectional area (CSA) of muscle fibers than the SR and PNR groups. Six weeks after repair, the PR and PNR groups showed more adipose cells, less CD68-stained cells, and more parallel tendon collagen fibers than the SR group. The PR group had more CD 68-stained cells than the PNR group. The PR group showed a larger CSA than the SR group. The mean load-to-failure values of the PR and PNR groups were higher than that of the SR group, although these differences were not significant.
CONCLUSION: PDRN and PN may improve tendon healing and decrease fatty degeneration after cuff repair.
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KEYWORD
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Polydeoxyribonucleotide, Polynucleotide, Tendon healing, Fatty degeneration, Chronic rotator cuff tear, Rat model
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